Abstract Nisin and reuterin are used in processed foods as preservatives. They are considered to be potential sources of natural antibiotics for the treatment of food contaminated by antibiotic-resistant bacteria, such as methicillin-resistantStaphylococcus aureus. We try to compare the best effect of both nisin and reuterin on the growth of methicillin-resistant S. aureus (MRSA) and S. aureus ATCC 25923. The results indicated that nisin at different concentrations inhibited the growth of methicillin-resistant S. aureus after 4 h, whereas after 12 and 24 h, the bacteria have regrown and reactivated. S. aureus ATCC 25923 was not affected by nisin concentration and has continuously grew at all times. Reuterin at different concentrations gradually inhibited the growth of methicillin-resistant S. aureus and S. aureus ATCC 25923 and declined after 12 h for both. Therefore, it can be deduced that nisin alone exerts a bacteriostatic effect on methicillin-resistant S. aureus but not on S. aureus ATCC 25923. Reuterin is considered as bactericidal effect on both microorganisms. The combination of nisin at a concentration of 25.6 and reuterin at a concentration of 5.2 mg/mL exerted a bactericidal effect on both microorganisms. By mixing nisin with reuterin, it can be more active against both microorganisms.

Abstract Breast cancer is the greatest common malignancy in females. This syndrome represents a serious public health problem that needs additional study to define its prediction and specific treatment. MCF-7 is a usually used breast cancer cell line that has been spread for many years by multiple groups. It shows to be a suitable model cell line for breast cancer studies worldwide, including those concerning anticancer. Anticancer and anti-inflammatory influences are important to treat the threats of chronic inflammation related with chronic diseases. Natural products have made and are remaining to make vital influences to the search for new anticancer agents. The use of natural foodstuffs in relationship with green nanoparticles has drawn attention because of its easy and eco-friendly protocol. Therefore, we aimed to investigate the antiproliferative activity of silver nanoparticles biosynthesized by mangosteen peel extract (MPE) on human breast cancer cell line (MCF-7) using [3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) method. In the present study serial concentrations (5, 10, 15, 50, 70, µg/mL) of cells biosynthesized silver nanoparticles by (MPE) was tested against MCF-7 cell lines showed a cytotoxic effects, morphological degeneration, damaging and has more effectiveness against breast cancer cells. The results showed a substantial a high reducing power, a sensible antiproliferative role and antioxidant activity. The results showed that silver nanoparticles biosynthesized by mangosteen peel extract (MPE) can be regarded as a suitable candidate for designing anticancer pharmaceutical preparations.

Abstract The deterioration of fish by microorganisms is very quickly, because it’s a highly perishable foods and short shelf life, for this reason the handling and stored condition very important to slow down and extend the shelf life and its quality. There are a number of strategies used as traditional methods to extend the shelf life of fishes included: chilling, freezing, icing, drying, smoking and salting. From the innovative processing technologies using of icing system with organic acids is considered to be a preservative method and delaying the changing in the fish and able to guarantee the quality with extension of their shelf-life. The present study provides the addition of organic acid as lactic acid (0.1%), propylene glycol (7%), potassium sorbate (1000 ppm) and mixture of potassium sorbate (1000 ppm) + sodium benzoate (0.2%) to fresh water and dipping the mullet fish in it then preserved in fresh water crushed ice medium containing the same concentration of organic acids. Different parameters were determined to limit the quality and safety of mullet during storage periods as total volatile nitrogen base, trimethylamine, thiobarbituric acid-value, refractive index of the muscle fluids, electrical resistance of the fish muscles, organoleptic evaluations and in addition to total viable count of bacteria and psychrophilic bacteria. The mixture of potassium sorbate and sodium benzoate using icing system method extended the shelf life of mullet fish up to 17-19 days of storage period in comparison with control and other organic acids.